MasterBeads Streptavidin/ StreptaDivin
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The very high binding affinity of the streptavidin-biotin interaction (Kd=10^-15) is used in a vast number of applications such as diagnostic assays, biomedical research, cell separation and nucleic acids research. Different applications require different bead and the choice depends on the final assay. To allow flexibility, Ademtech has engineered different surfaces of Streptavidin Adembeads along with differents sizes and binding capacities


 

Ademtech magnetic nanoparticles  (100nm, 200nm, 300nm, 500nm) offer large surface area for binding and eliminate the sedimentation problems that affect larger particles. Adembeads present a smooth surface exhibiting a low non–specific binding on the opposite of other available rough particles. Thanks to  the high iron content (> 70%) the rapid separation under magnetic field is not compromised. To ensure reliability of your assay, the tightly controlled production processes provide with a  calibrated (CV<20%)  spherical nanoparticles with highly reproducible characteristics.

CHOOSE THE IDEAL MAGNETIC NANOPARTICLES TO YOUR APPLICATION


MasterBeads, 500nm have an improved magnetic response and keep all benefits of Adembeads (no sedimenation) and are particularly suitable for automation.

MasterBeads are available pre-coated with

o  Streptavidin: natural streptavidin from S. avidinii

o  StreptaDivin: neutralized avidin from eggs without carbohydrates and tripeptide sequence Arg–Tyr–Asp (RYD).
The StreptaDivin has a isolectric point of 6.3. These features provide very low non-specific binding

available in 100nm, 200nm, 300nm! see HERE
9 differents streptavidin particles !

 

MasterBeads Streptavidin are supplied at 1% (10mg/ml) in Storage Buffer (#10201).
Immobilisation Buffer (#10301) can be used for biotinylated ligand capture

References:

1 Sensors & Actuators: B. Chemical, 134 (2), p.755-760, Sep 2008, An impedimetric DNA sensor based on functionalizd magnetic nnoparticles for HIV and HBV detection Hassen, W.M. / Chaix, C. / Abdelghani, A. / Bessueille, F. / Leonard, D. / Jaffrezic-Renault, N.
 
2-Electrochemistry Communications
, 10 (8), p.1152-1154, Aug 2008, A conductometric immunosensor based on functionalized magnetite nanoparticles for E. coli detection Hnaiein, M. / Hassen, W.M. / Abdelghani, A. / Fournier-Wirth, C. / Coste, J. / Bessueille, F. / Leonard, D. / Jaffrezic-Renault, N.
 
3-Materials Science & Engineering C, 28 (5), p.820-825, Jul 2008, Under flow impedimetric measurements using magnetic particles for label-free detection affinity target, Hassen, W.M. / Angnes, L. / Abdelghani, A. / Bessueille, F. / Leonard, D. / Jaffrezic-Renault, N.
 
4-Immunology Letters Volume 125, Issue 2 15 August 2009, Pages 100-104  Exogenous HIV-1 Vpr disrupts IFN-α response by plasmacytoid dendritic cells (pDCs) and subsequent pDC/NK interplay Henoch Sangjoon Hong, Nupur Bhatnagar, Matthias Ballmaier, Ulrich Schubert, Peter Henklein, Thorsten Volgmann, Hans eiken, Reinhold E. Schmidt and Dirk Meyer-Olson
 
5- BioTechniques 46:543-549 (June 2009) doi 10.2144/000113124 Simple non-fluorescent polarity labeling of microtubules for molecular motor assays Virupakshi Soppina, Arpan K. Rai, and Roop Mallik

6-Biosensors and Bioelectronics, 24 (7), p.1893-1898, Mar 2009, Rapid DNA multi-analyte immunoassay on a magneto-resistance biosensor Koets, M. / van der Wijk, T. / van Eemeren, J.T.W.M. / van Amerongen, A. / Prins, M.W.J


 
 
 
 Bio-Adembeads Streptavidin / Plus / Divin / Rec
 
 Immobilisation Buffer
 
 Adem-Mag SV
 Adem-Mag MV
 Adem-Mag HV
 Adem-Mag 96
 Adem-Mag MODULO
 
 MasterBeads Carboxylic Acid
 Activ-MasterBeads
 
 Brochure
 Protocol MasterBeads Streptavidin
 Protocol MasterBeads StreptaDivin
ADEMTECH - BioParc BIOGALIEN - Bat C 1 étage - 27 allée Charles DARWIN - 33600 PESSAC - France
Ph: +33(0)557.020.201 - Fax: +33(0)557.020.206 - e-mail : ademtech@ademtech.com