Bio-Adembeads Streptavidin / Plus / Divin / Rec
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The very high binding affinity of the streptavidin-biotin interaction (Kd=10^-15) is used in a vast number of applications such as diagnostic assays, biomedical research, cell separation and nucleic acids research. Different applications require different bead and the choice depends on the final assay. To allow flexibility, Ademtech has engineered different surfaces of Streptavidin Adembeads along with differents sizes and binding capacities.


 

Ademtech magnetic nanoparticles  (200nm, 300nm, 500nm) offer large surface area for binding and eliminate the sedimentation problems that affect larger particles. Adembeads present a smooth surface exhibiting a low non–specific binding on the opposite of other available rough particles. Thanks to  the high iron content (> 70%) the rapid separation under magnetic field is not compromised. To ensure reliability of your assay, the tightly controlled production processes provide with a  calibrated (CV<20%)  spherical nanoparticles with highly reproducible characteristics.


CHOOSE THE IDEAL MAGNETIC NANOPARTICLES TO YOUR APPLICATION

LARGE CHOICE OF SIZE  

200nm and 300nm respectively provide with a  15 m2/g and 10 m2/g
 
o  MasterBeads, 500nm provide with a 5 m2/g surface. MasterBeads have an improved magnetic response and keep all benefits of Adembeads (no sedimenation) and are particularly suitable for automation. See the Masterbeads Streptavidin.

LARGE CHOICE OF BINDING CAPACITIES = 5 levels of biotin-binding capacities are now available to choose the capacity needed to optimize your application.
 
 
LARGE CHOICE OF SURFACE = Adembeads are available pre-coated with
o  Streptavidin: natural streptavidin from S. avidinii

o  Streptavidin plus: natural streptavidin from S. Avidinii with improved binding capacities

o  StreptaDivin: neutralized avidin from eggs without carbohydrates and tripeptide sequence Arg–Tyr–Asp (RYD).
The StreptaDivin has a isolectric point of 6.3. These features provide very low non-specific binding.

o  rec-Streptavidin: recombinant streptavidin from E. Coli
also available in 500nm ! see HERE
9 differents streptavidin particles !

 

Streptavidin particles are suplied in Storage Buffer (#10201) at 0,5% (5mg/ml)
Immobilisation Buffer (#10301) can be used for biotinylated ligand capture.

References:

1 Sensors & Actuators: B. Chemical, 134 (2), p.755-760, Sep 2008, An impedimetric DNA sensor based on functionalizd magnetic nnoparticles for HIV and HBV detection Hassen, W.M. / Chaix, C. / Abdelghani, A. / Bessueille, F. / Leonard, D. / Jaffrezic-Renault, N.
 
2-Electrochemistry Communications, 10 (8), p.1152-1154, Aug 2008, A conductometric immunosensor based on functionalized magnetite nanoparticles for E. coli detection Hnaiein, M. / Hassen, W.M. / Abdelghani, A. / Fournier-Wirth, C. / Coste, J. / Bessueille, F. / Leonard, D. / Jaffrezic-Renault, N.
 
3-Materials Science & Engineering C, 28 (5), p.820-825, Jul 2008, Under flow impedimetric measurements using magnetic particles for label-free detection affinity target, Hassen, W.M. / Angnes, L. / Abdelghani, A. / Bessueille, F. / Leonard, D. / Jaffrezic-Renault, N.
 
4-Immunology Letters Volume 125, Issue 2 15 August 2009, Pages 100-104  Exogenous HIV-1 Vpr disrupts IFN-α response by plasmacytoid dendritic cells (pDCs) and subsequent pDC/NK interplay Henoch Sangjoon Hong, Nupur Bhatnagar, Matthias Ballmaier, Ulrich Schubert, Peter Henklein, Thorsten Volgmann, Hans eiken, Reinhold E. Schmidt and Dirk Meyer-Olson
 
5- BioTechniques 46:543-549 (June 2009) doi 10.2144/000113124 Simple non-fluorescent polarity labeling of microtubules for molecular motor assays Virupakshi Soppina, Arpan K. Rai, and Roop Mallik

6-Biosensors and Bioelectronics, 24 (7), p.1893-1898, Mar 2009, Rapid DNA multi-analyte immunoassay on a magneto-resistance biosensor Koets, M. / van der Wijk, T. / van Eemeren, J.T.W.M. / van Amerongen, A. / Prins, M.W.J


 
 
 
 Storage Buffer
 Immobilisation Buffer
 
 Adem-Mag SV
 Adem-Mag MV
 Adem-Mag HV
 Adem-Mag 96
 Adem-Mag MODULO
 
 Streptavidin and StreptaDivin
 
 Brochure
 Protocol Bio-Adembeads Streptavidin 200
 Protocol Bio-Adembeads Streptavidin 300
 Protocol Bio-Adembeads Streptavidin plus 200
 Protocol Bio-Adembeads Streptavidin plus 300
 Protocol Bio-Adembeads StreptaDivin
 Protocol Bio-Adembeads rec-Streptavidin
 QP DNA/RNA Binding Protein Isolation English
 QP DNA/RNA Binding Protein Isolation French
 QP Nucleic Acid Isolation English
 QP Nucleic Acid Isolation French
 POSTER ASM 2010
ADEMTECH - BioParc BIOGALIEN - Bat C 1 étage - 27 allée Charles DARWIN - 33600 PESSAC - France
Ph: +33(0)557.020.201 - Fax: +33(0)557.020.206 - e-mail : ademtech@ademtech.com