The ChIP-Adem-Kit is an innovative system especially designed for monitoring transcription factors or histones / DNA interactions. The ChIP-Adembeads Protein A/G after incubation with the Blocking Buffer have a specific surface designed for efficient Protein / DNA complex immunoprecipitation and to minimize non-specific enrichment. Proteins and other contaminants are eliminated in the washing steps. The ChIP protocol is greatly simplified by using blocked Protein A/G-coated nanoparticles. Some steps have been completely eliminated. Unlike traditional agarose beads, chromatin pre-clearing is not required. ChIP-Adembeads combined to designed buffers reduce background and maximize the amount of templates available for the reaction, achieving greater sensitivity where maximum DNA recovery is critical. The isolated DNA can be used in downstream applications : PCR and Real-Time PCR.Ademtech announced the availability of the new version of ChIP-Adembeads Protein A/G (#04242/04342) and ChIP Adem-kit Protein A/G (#04243/04343). These new kits offer several advantages over the current CHIP kits:
· User friendly: new Blocking Buffer colorless
· New Washing Buffer: Improved formulation with no stringent salt as LiCl reduced magnetic beads aggregation. Magnetic particles are easy to resuspend during washing steps.
ChIP-Adembeads Protein A/G (#04240, 04340) and ChIP-Adem-Kit (#04241, 04341) have been superseded by an Updated version. These products may still be ordered as long as inventory is available. These products are schedule to be discontinued at May 2012. We apologize for any inconvenience and hope this transition period will allow you to complete any ongoing projects
References for ChIP-Adembeads Protein A/G1- Genes & Dev., 2009, 23: 1399-1407. Metabolic intermediates selectively stimulate transcription factor interaction and modulate phosphate and purine pathways. Benoît Pinson, Sabine Vaur, Isabelle Sagot, Fanny Coulpier, Sophie Lemoine, Bertrand Daignan-Fornier.
2- Molecular and Cellular Biology, March 2010, p.1145-1157, Vol. 30, no. 5. Splicing Factor Spf30 Assists Exosome-Mediated Gene Silencing in Fission Yeast. Pascal Bernard, Julie Drogat, Sonia Dheur, and Jean-Paul Javerzat. 3- PNAS, May 2010, p.8416–8421, vol. 107, no. 18. Estradiol inhibits ongoing autoimmune neuroinflammation and NFκB-dependent CCL2 expression in reactive astrocytes. Sébastien N. Giraud, Cécile M. Caron, Danielle Pham-Dinh, Patrick Kitabgi, and Arnaud B. Nicot. 4- Cell, August 2011, 146, p.372-383. Spo11-Accessory Proteins Link Double-strand Break Sites to the Chromosome Axis in Early Meiotic Recombination. Panizza S, Mendoza M, Berlinger M, Huang L, Nicolas A, Shirahige K, Klein F.
5- Mol Cell Biol. 2011 April; 31(8): 1771–1786. Psm3 Acetylation on Conserved Lysine Residues Is Dispensable for Viability in Fission Yeast but Contributes to Eso1-Mediated Sister Chromatid Cohesion by Antagonizing Wpl1 Amélie Feytout, Sabine Vaur, Sylvie Genier, Stéphanie Vazquez, and Jean-Paul Javerzat
6-Molecular and Cellular Biology (2011) Volume: 31, Issue: 5, Pages:1088-1097 Role for cohesin in the formation of a heterochromatic domain at fission yeast subtelomeres, Sonia Dheur, Sven J Saupe, Sylvie Genier, Stéphanie Vazquez, Jean-Paul Javerzat